#SERIAL BASICS FREE FR SERIAL CLONER SERIAL#
Gateway cloning for both BP and LR reactions is easier with Serial Cloner, and it also provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server and extract a consensus a virtual cutter, a web browser with instant import of NCBI/EMBL entries, and a silent restriction map generator. shRNA constructions based on pre-defined scaffolds are automated, while assembling fragments obtained by PCR, adaptor/shRNA synthesis, or graphically selecting fragments between restriction sites is simplified with just a few clicks. Users can also set up PCR-based amplification quickly or prepare synthetic adaptors. It provides classical restriction maps, both graphic and text-based, as well as site usage windows. Using Serial Cloner, users can set up new sub-cloning projects and prepare electronic versions of constructs. The graphic map of Serial Cloner provides a truly graphic interface, where users can easily select and extract a feature, fragment, or show single, double or multiple cutters all in the same window. Serial Cloner also allows users to build text restriction maps, quickly formatting them to add multi-frame translation, or showing single cutters, for example. All the tools needed for analyzing and manipulating sequences are contained in a single all-in-one-window concept, with key functions easily accessible via the compact interface.
#SERIAL BASICS FREE FR SERIAL CLONER SOFTWARE#
The software provides powerful graphical display tools to facilitate analysis and construction processes in an intuitive way. Genbank/EMBL files can be imported or read from the internet via the included web interface. The new version allows for ApE and VectorNTI files to be read with their features, as well as pDRAW32 sequences.
Serial Cloner is compatible with DNA Strider files, as well as the import and export of files in FASTA format. Users can annotate any sequence portion, or the automatic scan feature can be used to detect common features. One of the most requested features for Serial Cloner was the ability to handle Features/Annotations, and this enhancement has now been fully implemented.
The software boasts an intuitive interface that makes working with complex biological data easier. Serial Cloner has been designed with molecular biology scientists in mind, as it offers powerful tools to assist with DNA cloning, sequence analysis, and visualization.
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